One-pot fermentation for erythritol production from distillers grains by the co-cultivation of Yarrowia lipolytica and Trichoderma reesei. (May 2022)

• Record Type: Journal Article
• Authors: Liu, Xiaoyan; Yu, Xinjun; He, Aiyong; Xia, Jun; He, Jianlong; Deng, Yuanfang; Xu, Ning; Qiu, Zhongyang; Wang, Xiaoyu; Zhao, Pusu
• Is Part Of: Bioresource technology. Volume 351(2022)
• Publication Date: 2022-05
• Subjects: Co-cultivation -- Erythritol production -- Cellulase -- Distillers grain -- Condition optimization; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.127053
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Co-culture of Y. lipolytica and T. reesei is first proposed for producing erythritol. One-pot SSF using DGS as feedstock is established on optimized co-culture conditions. Erythritol is produced from the in-situ cellulase hydrolyzed DGS in co-cultivation. An enhanced erythritol production of 267.1 mg/gds is achieved via the one-pot SSF. Abstract: A co-fermentation process involving Yarrowia lipolytica and Trichoderma reesei was studied, using distillers grains (DGS) as feedstocks for erythritol production. DGS can be effectively hydrolyzed by cellulase in the single-strain culture of T. reesei . One-pot solid state fermentation for erythritol production was then established by co-cultivating Y. lipolytica M53-S with the 12 h delay inoculated T. reesei Rut C-30, in which efficient saccharification of DGS and improved production of erythritol were simultaneously achieved. The 10:1 inoculation proportion of Y. lipolytica and T. reesei contributed to the maximum erythritol production of 267.1 mg/gds under the optimal conditions including initial moisture of 55%, pH of 5.0, NaCl addition of 0.02 g/gds and DGS mass of 200 g in 144 h co-cultivation. Being compared with the attempts to produce erythritol from other raw materials, the one-pot SSF with DGS is proposed to be a potential strategy for efficient and economical erythritol production.