Recent Technologies for Genetic Code Expansion and their Implications on Synthetic Biology Applications. Issue 8 (30th April 2022)

• Record Type: Journal Article
• Authors: Tang, Hongting; Zhang, Pan; Luo, Xiaozhou
• Is Part Of: Journal of molecular biology. Volume 434:Issue 8(2022)
• Publication Date: 2022-04-30
• Subjects: non-canonical amino acid biosynthesis -- GCE optimization -- synthetic parts -- synthetic circuits -- synthetic chassis; Molecular biology -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Bacteriology -- Periodicals; Molecular Biology -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biologie -- Périodiques; Biochimie -- Périodiques; Moleculaire biologie; Biochemistry; Biology; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.jmb.2021.167382
• Languages: English
• ISSNs: 0022-2836

Abstract

Graphical abstract: Highlights: Genetic code expansion is optimized by synthetic biology. Biosynthesis of non-canonical amino acids using cell factories. Creation of new synthetic parts, circuits, and chassis by genetic code expansion. Abstract: Genetic code expansion (GCE) enables the site-specific incorporation of non-canonical amino acids as novel building blocks for the investigation and manipulation of proteins. The advancement of genetic code expansion has been benefited from the development of synthetic biology, while genetic code expansion also helps to create more synthetic biology tools. In this review, we summarize recent advances in genetic code expansion brought by synthetic biology progresses, including engineering of the translation machinery, genome-wide codon reassignment, and the biosynthesis of non-canonical amino acids. We highlight the emerging application of this technology in construction of new synthetic biology parts, circuits, chassis, and products.