Immunological effect of fucosylated chondroitin sulfate and its oligomers from Holothuria fuscogilva on RAW 264.7 cells. (1st July 2022)

• Record Type: Journal Article
• Authors: Gong, Pi-Xian; Wu, Yan-Chao; Chen, Xiao; Zhou, Ze-Lin; Chen, Xi; Lv, Shi-Zhong; You, Yue; Li, Hui-Jing
• Is Part Of: Carbohydrate polymers. Volume 287(2022)
• Publication Date: 2022-07-01
• Subjects: Holothuria fuscogilva -- Fucosylated chondroitin sulfate -- RAW 264.7 -- Immunoregulatory; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.119362
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Fucosylated chondroitin sulfate was obtained from the sea cucumber Holothuria fuscogilva (FCS hf ). The structure was elucidated by NMR and HILIC-FTMS analysis. FCS hf contained a chondroitin core chain [→3)-β-D-GalNAc-(1 → 4)-β-D-GlcA-(1→]n, where the sulfation positions were the O-4 or O-6 of the GalNAc residues. The ratio of sulfated and non-sulfated GalNAc at O-6 was 1:2, while the ratio of GalNAc at O-4 was 1:1. 2, 4-disulfated-fucose (Fuc2, 4S), 4-sulfated-fucose (Fuc4S) and 3, 4-disulfated-fucose (Fuc3, 4S) were attached to the O-3 of GlcA with a molar ratio of 1.00: 0.62: 1.32. The FCS hf could significantly promote the proliferative rate, NO production and neutral red uptake of RAW 264.7 cells within the concentration range of 10–300 μg/mL. Compared with the fucosylation and deacetylation degrees, the molecular weight of FCS hf had markedly influence on the activation of RAW 264.7 cells. A decrease in molecular weight dramatically improved the immunoregulatory activities. Furthermore, FCS hf activated RAW 264.7 cells through TLR-2/4-NF-κB pathway. Graphical abstract: Unlabelled Image