Decolorization and detoxification of synthetic dye compounds by laccase immobilized in vault nanoparticles. (May 2022)

• Record Type: Journal Article
• Authors: Gao, Yifan; Wang, Meng; Shah, Kshitija; Singh Kalra, Shashank; Rome, Leonard H.; Mahendra, Shaily
• Is Part Of: Bioresource technology. Volume 351(2022)
• Publication Date: 2022-05
• Subjects: Protein nanocages -- Ligninolytic -- Immobilization -- Decolorization -- Cytotoxicity; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.127040
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Fungal laccases were characterized, produced, and encapsulated in vault nanocages. Thermal and pH stability of laccase notably improved by vault packaging (vlaccase). vLaccase was superior to natural laccase in decolorizing and detoxifying dyes. vLaccase treatment greatly reduced toxicity of synthetic dyes degradation products. Abstract: This study presents an eco-friendly and efficient technology, using immobilized enzymes, vault-encapsulated laccases (vlaccase), for decolorization and detoxification of dyes. Vault encapsulation remarkably improved the performance of laccase at industrially relevant conditions, including neutral to alkaline pH and relatively high temperatures. Two representative anthraquinone and azo dyes, Reactive Blue 19 and Acid Orange 7, respectively, were rapidly decolorized (72% and 80%) by vlaccase treatment while natural laccase (nlaccase) achieved 40% and 32% decolorization. The toxicity of treated and untreated dyes was tested on model bacterial, algal, and insect cells. The inhibitory effects of dyes towards selected bacteria were reduced in vlaccase-treated samples. The chlorophyll synthesis in algae was less inhibited by dyes after vlaccase treatment. Furthermore, the toxicity of dye degradation products to insect cells was significantly mitigated in the vlaccase group. Collectively, these results indicate that vlaccase is a stable and strong enzymatic system for removing dyes from waters.