Chitosan and carboxymethyl chitosan mimic biomineralization and promote microbially induced calcium precipitation. (1st July 2022)

• Record Type: Journal Article
• Authors: Wang, Zhao; Su, Junfeng; Ali, Amjad; Yang, Wenshuo; Zhang, Ruijie; Li, Yifei; Zhang, Lingfei; Li, Jiawei
• Is Part Of: Carbohydrate polymers. Volume 287(2022)
• Publication Date: 2022-07-01
• Subjects: Chitosan -- Carboxymethyl chitosan -- Microbially induced calcium precipitation -- Nucleation sites -- Intensified strategies; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.119335
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: This research aimed to evaluate chitosan (CTS) and carboxymethyl chitosan (CMCS) as polysaccharides that could mimic the role of bacterial extracellular polymeric substance (EPS) in the biomineralization process through bionic experiments. The introduction of COOH resulted in higher calcium precipitation efficiency of CMCS (65.07%) than CTS (55.66%). CaCO3 nucleation on the surface of CTS and CMCS was triggered through the binding of Ca 2+ to NH2, OH, COOH and NHCOCH3 groups. Moreover, the experiment of polysaccharides mediated biomineralization was conducted. The maximum calcium precipitation efficiency reached 96.07% with the addition of 0.15% CMCS. Combining the characterization results, the synergetic mineralization mechanisms between polysaccharides and bacteria were proposed. Among them, bacterial metabolic by-products (alkalinity), active groups and adhesion of polysaccharides played a significant role. This work provides a reference for further understanding of the biomineralization mechanism, and gives a new insight into the intensified strategies of MICP technology. Graphical abstract: Unlabelled Image Highlights: Polysaccharides may act as the nucleation sites in the biomineralization process. The feasibility of polysaccharides and bacteria co-mineralization was assessed. CMCS had a higher compatibility as compared to CTS with strain WZ39. The MICP efficiency was improved by 28.28% with the addition of 0.15% CMCS. This work provides a new insight into the intensified strategies of MICP technology.