Comparative analysis of antibiotic resistance genes on a pig farm and its neighboring fish ponds in a lakeside district. (15th June 2022)

• Record Type: Journal Article
• Authors: Fu, Chenxi; Ding, Huijun; Zhang, Qianqian; Song, Yaqiong; Wei, Yuguang; Wang, Yao; Wang, Boming; Guo, Jiaxuan; Qiao, Min
• Is Part Of: Environmental pollution. Volume 303(2022)
• Publication Date: 2022-06-15
• Subjects: Antibiotic resistance genes -- Swine wastewater -- Water quality indicators; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2022.119180
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Antibiotics usage in animal production is considered a primary driver of the occurrence, supply and spread of antibiotic resistance genes (ARGs) in the environment. Pig farms and fish ponds are important breeding systems in food animal production. In this study, we compared and analyzed broad ARGs profiles, mobile genetic elements (MGEs) and bacterial communities in a representative pig farm and neighboring fish ponds around Poyang Lake, the largest freshwater lake in China. The factors influencing the distribution of ARGs were also explored. The results showed widespread detection of ARGs (from 57 to 110) among 283 targeted ARGs in the collected water samples. The differences in the number and relative abundance of ARGs observed from the pig farm and neighboring fish ponds revealed that ARG contamination was more serious on the pig farm than in the fish ponds and that the water treatment plant on the pig farm was not very effective. Based on the variance partition analysis (VPA), MGEs, bacterial communities and water quality indicators (WIs) codrive the relative abundance of ARGs. Based on network analysis, we found that total phosphorus and Tp614 were the most important WIs and MGEs affecting ARG abundance, respectively. Our findings provide fundamental data on farms in lakeside districts and provide insights into establishing standards for the discharge of aquaculture wastewater. Graphical abstract: Image 1 Highlights: Pig farms have more diverse bacteria and ARGs than fish ponds. The contents of phosphorus in water may cause different ARG distribution. Water treatment process of the pig farm may not effectively reduce ARGs.