Flow cytometric minimal residual disease assessment in B‐cell precursor acute lymphoblastic leukaemia patients treated with CD19‐targeted therapies — a EuroFlow study. (8th December 2021)

• Record Type: Journal Article
• Authors: Verbeek, Martijn W. C.; Buracchi, Chiara; Laqua, Anna; Nierkens, Stefan; Sedek, Lukasz; Flores‐Montero, Juan; Hofmans, Mattias; Sobral de Costa, Elaine; Nováková, Michaela; Mejstrikova, Ester; Barrena, Susana; Kohlscheen, Saskia; Szczepanowski, Monika; Kulis, Jan; Oliveira, Elen; Jugooa, Romana; de Jong, Anja X.; Szczepanski, Tomasz; Philippé, Jan; van Dongen, Jacques J. M.; Orfao, Alberto; Brüggemann, Monika; Gaipa, Giuseppe; van der Velden, Vincent H. J.
• Is Part Of: British journal of haematology. Volume 197:Number 1(2022)
• Page Start: 76
• Page End: 81
• Publication Date: 2021-12-08
• Subjects: acute leukaemia -- diagnostic haematology -- flow cytometry -- minimal residual disease; Hematology -- Periodicals; Blood -- Diseases -- Periodicals; 616.15
• DOI: 10.1111/bjh.17992
• Languages: English
• ISSNs: 0007-1048

Abstract

Summary: The standardized EuroFlow protocol, including CD19 as primary B‐cell marker, enables highly sensitive and reliable minimal residual disease (MRD) assessment in B‐cell precursor acute lymphoblastic leukaemia (BCP‐ALL) patients treated with chemotherapy. We developed and validated an alternative gating strategy allowing reliable MRD analysis in BCP‐ALL patients treated with CD19‐targeting therapies. Concordant data were obtained in 92% of targeted therapy patients who remained CD19‐positive, whereas this was 81% in patients that became (partially) CD19‐negative. Nevertheless, in both groups median MRD values showed excellent correlation with the original MRD data, indicating that, despite higher interlaboratory variation, the overall MRD analysis was correct.