Quantitative proteomics revealed new functions of ALKBH4. Issue 7 (5th January 2022)

• Record Type: Journal Article
• Authors: Yu, Kailin; Qi, Tianyu F.; Miao, Weili; Liu, Xiaochuan; Wang, Yinsheng
• Is Part Of: Proteomics. Volume 22:Issue 7(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-01-05
• Subjects: bisulfite sequencing -- cytosine methylation -- mass spectrometry -- parallel‐reaction monitoring -- post‐translational modification -- quantitative proteomics -- SILAC; Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.202100231
• Languages: English
• ISSNs: 1615-9853

Abstract

Abstract: ALKBH4 is a versatile demethylase capable of catalyzing the demethylation of monomethylated lysine‐84 on actin and N 6 ‐methyladenine in DNA. In this study, we conducted a quantitative proteomic experiment to reveal the altered expression of proteins in HEK293T cells upon genetic ablation of ALKBH4 . Our results showed markedly diminished levels of GSTP1 and HSPB1 proteins in ALKBH4‐depleted cells, which emanate from an augmented expression level of DNA (cytosine‐5)‐methyltransferase 1 (DNMT1) and the ensuing elevated cytosine methylation in the promoter regions of GSTP1 and HSPB1 genes. Together, our results revealed a role of ALKBH4 in modulating DNA cytosine methylation through regulating the expression level of DNMT1 protein.