3D Bioprinting of Multifunctional Dynamic Nanocomposite Bioinks Incorporating Cu‐Doped Mesoporous Bioactive Glass Nanoparticles for Bone Tissue Engineering. Issue 12 (1st February 2022)

• Record Type: Journal Article
• Authors: Zhu, Hui; Monavari, Mahshid; Zheng, Kai; Distler, Thomas; Ouyang, Liliang; Heid, Susanne; Jin, Zhaorui; He, Jiankang; Li, Dichen; Boccaccini, Aldo R.
• Is Part Of: Small. Volume 18:Issue 12(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-02-01
• Subjects: alginate -- bioprinting -- dynamic crosslinks -- gelatin -- hydrogels -- mesoporous bioactive glass -- nanocomposites; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.202104996
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: Bioprinting has seen significant progress in recent years for the fabrication of bionic tissues with high complexity. However, it remains challenging to develop cell‐laden bioinks exhibiting superior physiochemical properties and bio‐functionality. In this study, a multifunctional nanocomposite bioink is developed based on amine‐functionalized copper (Cu)‐doped mesoporous bioactive glass nanoparticles (ACuMBGNs) and a hydrogel formulation relying on dynamic covalent chemistry composed of alginate dialdehyde (oxidized alginate) and gelatin, with favorable rheological properties, improved shape fidelity, and structural stability for extrusion‐based bioprinting. The reversible dynamic microenvironment in combination with the impact of cell‐adhesive ligands introduced by aminated particles enables the rapid spreading (within 3 days) and high survival (>90%) of embedded human osteosarcoma cells and immortalized mouse bone marrow‐derived stroma cells. Osteogenic differentiation of primary mouse bone marrow stromal stem cells (BMSCs) and angiogenesis are promoted in the bioprinted alginate dialdehyde‐gelatin (ADA‐GEL or AG)‐ACuMBGN scaffolds without additional growth factors in vitro, which is likely due to ion stimulation from the incorporated nanoparticles and possibly due to cell mechanosensing in the dynamic matrix. In conclusion, it is envisioned that these nanocomposite bioinks can serve as promising platforms for bioprinting complex 3D matrix environments providing superior physiochemical and biological performance for bone tissue engineering. Abstract : A multifunctional nanocomposite bioink is designed incorporating amine‐functionalized copper‐doped mesoporous bioactive glass nanoparticles in oxidized alginate‐gelatin hydrogel. Shape fidelity and structural stability after extrusion bioprinting are demonstrated. Rapid spreading and high survival of human osteosarcoma and immortalized mouse bone marrow‐derived stroma cells are confirmed, and osteogenic differentiation of primary mouse bone marrow stromal stem cells is promoted, which is likely the result of ion stimulation from nanoparticles, rendering the bioink highly attractive for bone regeneration.