In vitro antifungal activity of cold atmospheric microwave plasma and synergistic activity against Malassezia pachydermatis when combined with chlorhexidine gluconate. Issue 2 (19th January 2022)

• Record Type: Journal Article
• Authors: Lee, Tae‐Hyun; Hyun, Jae‐Eun; Kang, Yeong‐Hun; Baek, Seung‐Joon; Hwang, Cheol‐Yong
• Is Part Of: Veterinary medicine and science. Volume 8:Issue 2(2022)
• Page Start: 524
• Page End: 529
• Publication Date: 2022-01-19
• Subjects: antifungal effects -- chlorhexidine gluconate -- cold atmospheric microwave plasma -- Malassezia pachydermatis -- synergism; Veterinary medicine -- Periodicals; Animal Diseases; Veterinary medicine; Periodicals; Periodicals; 636.08905
• DOI: 10.1002/vms3.719
• Languages: English
• ISSNs: 2053-1095

Abstract

Abstract: Background: The antifungal efficacy of cold atmospheric microwave plasma (CAMP) against Malassezia pachydermatis has not been to be evaluated. Objective: To examine the antifungal effects of CAMP against M. pachydermatis and its synergistic effects with chlorhexidine gluconate (CHX). Methods: A M. pachydermatis isolate was collected from a dog with otitis externa and Malassezia dermatitis at the Seoul National University Veterinary Medical Teaching Hospital. The antifungal effect was determined by applying CAMP to a M. pachydermatis isolate that was incubated for 3 days at 37°C. After 1, 2, 3 and 5 min of application, the efficacy of the plasma treatment was determined according to the number of colony forming units (CFUs). A mixture consisting of inoculum and CHX was applied to evaluate the synergistic effect of the plasma treatment in the same way. Results: The application of CAMP showed significant antifungal effects against M. pachydermatis . The antifungal effect of CAMP was enhanced by an increased exposure time and output power. The application of CAMP with 0.02% and 0.2% CHX resulted in lower survival rates against M. pachydermatis when compared with its sole application at 1 or 2 min. Conclusions: The study findings demonstrate that CAMP has a potential as a new antifungal option for M. pachydermatis and has synergistic antifungal effects with CHX in vitro. Clinical applications for CAMP are necessary to assess the antifungal efficacy for patients. Abstract : Cold atmospheric microwave plasma (CAMP) caused a significant antifungal effect against Malassezia pachydermatis and also showed a synergistic activity with chlorhexidine gluconate (CHX) against M. pachydermatis .