U1 small nuclear ribonucleoprotein is essential for early larval development in silkworm, Bombyx mori. (23rd October 2021)

• Record Type: Journal Article
• Authors: Zhang, Xiaoqian; Yang, Dehong; Yang, Xu; Zhang, Xinran; Zhang, Yong; Huang, Yongping; Zhang, Shengxiang; Liu, Zulian
• Is Part Of: Insect science. Volume 29:Number 2(2022)
• Page Start: 379
• Page End: 387
• Publication Date: 2021-10-23
• Subjects: CRISPR/Cas9 -- development -- ecdysone -- silkworm -- U1 snRNP; Insects -- Periodicals; Entomology -- Periodicals; 595.705
• DOI: 10.1111/1744-7917.12946
• Languages: English
• ISSNs: 1672-9609

Abstract

Abstract: U1 small nuclear ribonucleoproteins ( U1 snRNP ) associates with 5ʹ splice sites in the form of ribonucleoprotein particles and is highly conserved among species. The physiological functions of U1 snRNP in a lepidopteran model insect Bombyx mori is mostly unknown. Here, we showed that U1 snRNP plays an important role in the development of silkworm. Knockout of U1 snRNP in silkworm showed either delayed or stationary 1st instar larva development compared with the wild‐type group. U1 snRNP deletion mutants exhibited abnormal cellular phenotypes with enlarged cell nucleus, scant cytoplasm and enlarged nuclei. RNA‐seq analysis revealed that genes involved in metabolic pathway, biosynthesis of secondary metabolites and steroid hormone biosynthesis were significantly affected by U1 snRNP depletion. Taken together, our study suggests that U1 snRNP homeostasis plays an important role in silkworm development. Abstract : Significantly enriched gene ontology terms for biological process, cellular component and molecular function from the differentially expressed genes (DEGs). Log2 fold change more than or equal to 5 and P <0.05 were used as the cutoff criteria.