Process optimization for the synthesis of functionalized Au@AgNPs for specific detection of Hg2+ based on quality by design (QbD). Issue 15 (23rd March 2022)

• Record Type: Journal Article
• Authors: Zhang, Hui; Wang, Baoling; Liu, Xiaoyi; Zhang, Hongxu; Yao, Jiangyu; Gong, Xingchu; Yan, Jizhong
• Is Part Of: RSC advances. Volume 12:Issue 15(2022)
• Page Start: 9121
• Page End: 9129
• Publication Date: 2022-03-23
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d2ra01500e
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : The current study highlights the advantages of using the quality by design (QbD) approach to synthesise and optimize SERS substrates for the detection of Hg 2+ . Abstract : The current study highlights the advantages of using the quality by design (QbD) approach to synthesise and optimize SERS substrates for the detection of Hg 2+ . Considering that the performance of Au@AgNPs is affected by many factors, Plackett–Burman (PB) experimental design was used to determine the critical process parameters (CPPs) for evaluating the performance of Au@AgNPs. The quantitative relationships between the CPPs and the critical quality attributes (CQAs) were assessed by Box-Behnken Design (BBD). The optimal design space for Au@AgNPs was calculated via a Monte Carlo algorithm. Finally, detection of Hg 2+ in the range of 1 ∼ 100 ng mL −1 ( R 2 = 0.9891) was achieved by SERS in combination with 4, 4-bipyridine (Dpy) as signal molecules. The recoveries for licorice ranged from 83.53% to 92.96%. Specificity and practicality studies indicated that the method based on the QbD concept and design space not only met the optimal performance of Au@AgNPs but also improved the rapid detection of Hg 2+ in Chinese medicine samples.