Identification of Rab family genes and functional analyses of LmRab5 and LmRab11A in the development and RNA interference of Locusta migratoria. (4th August 2021)

• Record Type: Journal Article
• Authors: Abbas, Mureed; Fan, Yun‐He; Shi, Xue‐Kai; Gao, Lu; Wang, Yan‐Li; Li, Tao; Cooper, Anastasia M.W.; Silver, Kristopher; Zhu, Kun Yan; Zhang, Jian‐Zhen
• Is Part Of: Insect science. Volume 29:Number 2(2022)
• Page Start: 320
• Page End: 332
• Publication Date: 2021-08-04
• Subjects: gene expression patterns -- Locusta migratoria -- Rab family genes -- RNA interference -- RNAi efficiency; Insects -- Periodicals; Entomology -- Periodicals; 595.705
• DOI: 10.1111/1744-7917.12921
• Languages: English
• ISSNs: 1672-9609

Abstract

Abstract: Rab proteins constitute the largest family of small GTPases, which play pivotal roles in intracellular membrane trafficking in all eukaryotes. A number of Rab genes have been identified in eukaryotes; however, very little information about these genes has been reported in insects. In the current study, for the first time we identified and characterized 27 Rab family genes from Locusta migratoria . Phylogenetic analysis and comparison of domain architecture indicated that Rab family genes are highly conserved among insect species. Tissue‐dependent expression profiles indicated that expression of Rab genes was highest in the ovary, except for LmRab3, which was most highly expressed in hemolymph. The biological function of each Rab gene was investigated using RNA interference (RNAi). Double‐stranded RNA targeting each Rab gene was injected into the hemocoel of nymphs and revealed that suppression of two Rab genes ( LmRab5 and LmRab11A ) caused 100% mortality. In addition, nymphs injected with ds LmRab5 exhibited severe phenotypic defects in the gastric caeca and midgut, while ds LmRab11A arrested the molting process. We then applied the RNAi of RNAi technique to test if silencing either of these two genes would affect the suppression of the lethal giant larvae ( LmLgl) reporter gene and found that suppression of LmRab5 diminished the RNAi efficiency of LmLgl, whereas suppression of LmRab11A enhanced RNAi efficiency of LmLgl . These results indicate that Rab genes contribute differently to RNAi efficiency in different tissues. Our study provides a foundation for further functional investigations of Rab genes and their contributions to RNAi efficiency in L. migratoria . Abstract : Graphical Abstract: A total of 27 Rab genes were identified and characterized in Locusta migratoria . Injection of dsRNAs targeting LmRab5 and LmRab11A caused 100% nymphal mortality, and exhibited severe midgut defects and arrested the molting process, respectively.