Copy number variation of the CCDC39 gene is associated with growth traits in Chinese cattle. Issue 2 (2nd March 2022)

• Record Type: Journal Article
• Authors: Hu, Linyong; Yu, Junjian; Huang, Rong; Yang, Peng; Zhang, Zijing; Chai, Yanan; Shi, Qiaoting; Chen, Fuying; Liu, Xian; Li, Zhiming; Ru, Baorui; Wang, Eryao; Lei, Chuzhao; Peng, Wei; Huang, Yongzhen
• Is Part Of: Veterinary medicine and science. Volume 8:Issue 2(2022)
• Page Start: 917
• Page End: 924
• Publication Date: 2022-03-02
• Subjects: association -- CCDC39 gene -- Chinese beef cattle -- copy number variations (CNV) -- growth trait -- mRNA expression; Veterinary medicine -- Periodicals; Animal Diseases; Veterinary medicine; Periodicals; Periodicals; 636.08905
• DOI: 10.1002/vms3.712
• Languages: English
• ISSNs: 2053-1095

Abstract

Abstract: Background: Copy number variation (CNV) has become an essential part of genetic structural variation. Coiled‐coil domain containing 39 ( CCDC39 ) is a gene that related to the growth and development of organs and tissues. It is identified that it has a CNV region by animal genome resequencing. Objective: In this study, we detected the phenotypic traits and different distributions of CCDC39 gene copy numbers in five Chinese cattle breeds (Qinchuan (QC) cattle, Yunling (YL) cattle, Xianan (XN) cattle, Pinan (PN) cattle and Jiaxian (JX) cattle). Methods: Five hundred and six cattle were randomly selected for CNV distribution detection. Blood samples were taken and genomic DNA was extracted. Different tissues were obtained from adult ( n = 3) XN cattle, including heart, liver, kidney, skeletal muscle and lung. The genome qPCR experiment was performed with SYBR Green in triplicate. CDNA qPCR was used to detect the expression level of CCDC39 in different tissues and varieties. Using SPSS v20.0 software, the relationship between CCDC39 CNV and the growth traits of PN, XN, QC, NY and YL cattle breeds was analyzed by one‐way analysis of variance (ANOVA). Results: The results showed that the expression of CCDC39 in lung was higher than that in other tissues. The expression in liver and kidney was similar, but the expression in heart and muscle was less. It can be seen that the duplication type of QC cattle CCDC39 CNV is higher than the deletion or normal in the height at hip cross. The normal type of PN cattle in body length and hip width was better than duplication and deletion ( p < 0.05). In XN cattle, the deletion type of CNV had superior growth characteristics in heart girth and cannon bone circumference compared with the duplication type and the normal type ( p < 0.05). Conclusion: The study revealed a significant association between CNV of CCDC39 gene and growth traits in different Chinese cattle breeds. Abstract : We detected the different distributions of CCDC39 gene copy numbers in five Chinese cattle breeds. We also detected the expression situation of CCDC39 gene in cattle. The study revealed the significant correlation between CNVs of CCDC39 gene and growth traits in different Chinese cattle breeds.