Cellulosimicrobium protaetiae sp. nov., isolated from the gut of the larva of Protaetia brevitarsis seulensis. Issue 3 (29th March 2022)

• Record Type: Journal Article
• Authors: Le Han, Ho; Nguyen, Tra T. H.; Li, Zhun; Shin, Na-Ri; Kim, Song-Gun
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 3(2022)
• Publication Date: 2022-03-29
• Subjects: Actinobacteria -- intestine of herbivorous insect; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005296
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-positive, non-spore-forming, yellow-pigmented, non-motile, non-flagellated, facultative anaerobic and rod-shaped bacterial strain, designated BI34 T, was isolated from the gut of the larva of Protaetia brevitarsis seulensis . Strain BI34 T grew at 15–40 °C (optimum, 37 °C), at pH 6.5–9.0 (optimum, pH 7.5) and in the presence of 0–7 % (w/v) NaCl (optimum, 2 %). Based on the results of 16S rRNA gene sequence analysis, strain BI34 T belonged to the phylum Actinobacteria and was closely related to Cellulosimicrobium funkei NBRC 104118 T (99.3 %), Cellulosimicrobium cellulans NBRC 15516 T (99.1 %), Cellulosimicrobium composti BIT-GX5 T (99.0 %), Cellulosimicrobium fucosivorans SE3 T (99.0 %), Cellulosimicrobium marinum NBRC 110994 T (98.4 %) and Cellulosimicrobium terreum DS-61 T (97.0 %). The genome to genome relatedness of the average nucleotide identity (ANI) and the digital DNA–DNA hybridization (dDDH) values calculated by the Genome-to-Genome Distance Calculator between strain BI34 T and its related species mentioned above were lower than the threshold of 95 and 70 % for speciation, respectively. The predominant menaquinone of strain BI34 T contained MK-9(H4 ), and the major fatty acids were anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0 . Strain BI34 T had diphosphatidylglycerol and phosphatidylglycerol as major polar lipids. The whole-cell sugars were galactose, glucose and ribose, and the cell-wall peptidoglycan contained lysine, alanine, aspartic acid and glutamic acid. The DNA G+C content of strain BI34 T was 73.8 mol%. The difference in physiological and biochemical characteristics and the below-threshold values of genome-to-genome relatedness indicate that strain BI34 T represents a novel species in the genus Cellulosimicrobium, for which the name Cellulosimicrobium protaetiae sp. nov. is proposed. The type strain is BI34 T (=KCTC 49302 T =NBRC 114073 T ).