Flavobacterium selenitireducens sp. nov., isolated from rhizosphere soil of ancient mulberry. Issue 3 (29th March 2022)

• Record Type: Journal Article
• Authors: Hou, Xiaoxiao; Li, Shucheng; Mao, Sidi; Mu, Weidong; Guo, Bai; Wei, Shuzhen; Huang, Mei; Zhao, Yanfang; Deng, Hongkuan; Sang, Feng; Chen, Zhiwei; Liu, Hongliang; Liu, Aijv
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 3(2022)
• Publication Date: 2022-03-29
• Subjects: genome -- phylogenomic tree -- Flavobacterium selenitireducens sp. nov -- mulberry -- rhizosphere; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005304
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, non-motile, aerobic, yellow, convex, rod-shaped mesophilic bacterial strain, designated strain D33 T, was isolated from rhizosphere soil of ancient mulberry in Dezhou city, Shandong province, PR China. The strain grew at 8–37 °C (optimum, 30 °C), pH 4–9 (optimum, pH 7) and growth occurred at 0.5–5.5 % (w/v) NaCl (optimally at 1 %). The results of the phylogenetic analyses of 16S rRNA gene and whole genome sequences indicated that D33 T was closely related to members of the genus Flavobacterium and had the highest 16S rRNA gene sequence similarity with ' Flavobacterium agri ' KACC 19300 (95.4 %), Flavobacterium ichthyis NST-5 T (94.6 %), Flavobacterium ahnfeltiae KCTC 32467 T (93.6 %) and Flavobacterium longum JCM 19141 T (93.6 %). The genome size of D33 T was 3.8 Mb and the DNA G+C content was 48.0 mol%. The average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH) and average amino acid identity (AAI) values among D33 T and reference strains were lower than the threshold values for species delineation. The only respiratory quinone of D33 T was menaquinone 6 (MK-6). The predominant fatty acids (>5 %) were C15:0, C16 : 0, C18 : 0, iso-C15:0, iso–C17 : 0 3-OH, anteiso-C15 : 0 and summed feature 9 . The polar lipid profile contained phosphatidylethanolamine, two unidentified aminophospholipids, three unidentified aminolipids and two unidentified lipids. Combined data from phenotypic, phylogenetic and chemotaxonomic studies indicated that D33 T is a representative of a novel species of the ge nus Flavobacterium, for which the name Flavobacterium selenitireducens sp. nov. is proposed. The type strain is D33 T (=GDMCC 1.1946 T =KACC 22131 T ).