A coumarin-based reversible two-photon fluorescence probe for imaging glutathione near N-methyl-d-aspartate (NMDA) receptors. Issue 22 (24th February 2022)

• Record Type: Journal Article
• Authors: Kwon, Nahyun; Lim, Chang Su; Lee, Dayoung; Ko, Gyeongju; Ha, Jeongsun; Cho, Moonyeon; Swamy, K. M. K.; Lee, Eun-Young; Lee, Dong Joon; Nam, Sang-Jip; Zhou, Xin; Kim, Hwan Myung; Yoon, Juyoung
• Is Part Of: Chemical communications. Volume 58:Issue 22(2022)
• Page Start: 3633
• Page End: 3636
• Publication Date: 2022-02-24
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc05512g
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : In this article, we report coumarin derivative 1 bearing cyanoacrylamide and ifenprodil moieties as the first reversible fluorescent probe that can monitor GSH near NMDA receptors. Abstract : Glutathione (GSH) is known to play a key role in the modulation of the redox environment in N -methyl-d-aspartate (NMDA) receptors. Coumarin derivative 1 bearing cyanoacrylamide and ifenprodil moieties was synthesized and reported to monitor GSH near NMDA receptors. The cyanoacrylamide moiety allows probe 1 to monitor GSH reversibly at pH 7.4 and the ifenprodil group acts as a directing group for NMDA receptors. Two-photon fluorescence microscopy allows probe 1 to successfully sense endogenous GSH in neuronal cells and hippocampal tissues with excitation at 750 nm. Furthermore, the addition of H2 O2 and GSH induced a decrease and an increase in fluorescence emission. Probe 1 can serve as a potential practical imaging tool to get important information on GSH in the brain.