Rrp14 controls rRNA transcription via facilitating the translocation of Pol5 into the nucleolus. Issue 5 (4th March 2022)

• Record Type: Journal Article
• Authors: Lin, Zhen; Liu, Haiyan; Chen, Hongzhi; Cao, Hongshi; Liu, Xiaochang; Zhu, Haichao; Zhao, Lu; Chen, Zhiming
• Is Part Of: Cell cycle. Volume 21:Issue 5(2022)
• Page Start: 489
• Page End: 500
• Publication Date: 2022-03-04
• Subjects: Schizosaccharomyces pombe -- rRNA transcription -- Rrp14 -- Pol5 -- nucleolus; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2021.2023303
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: Rrp14 is a conserved protein that plays an important role in rRNA processing and ribosomal biogenesis. In Schizosaccharomyces pombe, the rrp14 gene is split into SPAC8C9.10 c ( rrp14 ) and SPBC947.07 ( rrp1402 ). Although the SPAC8C9.10 c gene is not essential for S. pombe survival, deletion of the gene causes the yeast cells to grow sick and to exhibit decreased rRNA transcription. We identified a novel Pol5 protein that physically interacts with the Rrp14 protein. Taking advantage of the Pil1 co-tethering assay, we found that Rrp14 facilitates the nucleolus translocation of Pol5, and the 7-RINAWN-12 motif of the Rrp14 protein is responsible for the interaction between Pol5 and Rrp14. Since deletion of the 7-RINAWN-12 motif affects rRNA transcription, we thus propose that Rrp14 affects rRNA transcription by facilitating the nucleolus translocation of Pol5.