Acute toxicity and sublethal effects of nitrite on oxidative stress in early juvenile Brazilian flounder, Paralichthys orbignyanus. (22nd December 2021)

• Record Type: Journal Article
• Authors: Miranda, Diego Henrique de Sousa; Maltez, Lucas Campos; Campello, Marina Espírito Santo; Córdova, Joel Fitzgerald Linares; Rodrigues, Ricardo Vieira; Sampaio, Luís André; Okamoto, Marcelo Hideo
• Is Part Of: Aquaculture research. Volume 53:Number 5(2022)
• Page Start: 1939
• Page End: 1946
• Publication Date: 2021-12-22
• Subjects: antioxidant -- flounder -- marine fish -- nitrogenous compounds -- oxidative damage; Aquaculture -- Periodicals; Fishery management -- Periodicals; 639.8
• DOI: 10.1111/are.15722
• Languages: English
• ISSNs: 1355-557X

Abstract

Abstract: This study evaluated the acute toxicity and sublethal effects of nitrite on oxidative stress parameters in early juvenile Brazilian flounder, Paralichthys orbignyanus . Fish were exposed to six concentrations of nitrite: 20, 40, 80, 170, 330 and 550 mg NO2 − N‐L −1, plus a control with no added nitrite, all in triplicate for 96 h. Every 24 h, the tanks were monitored to check possible mortality to estimate the LC50 . At the end of the experiment, fish were sampled for stress oxidative analysis. All fish exposed to 550 mg NO2 − N‐L −1 died within the first 24 h. The LC50 ranged from 427.8 to 393.1 mg NO2 − N‐L −1 for 24 and 96 h respectively. The total antioxidant capacity against peroxyl radicals (ACAP) showed a gradual reduction in fish exposed to nitrite levels from 170 mg NO2 − N‐L −1 . The glutathione S‐transferase (GST) activity was higher in 80, 170 and 330 mg NO2 − N‐L −1 . The non‐proteic (NPSH) and proteic (PSH) thiols levels were higher in 170 and 330 mg NO2 − N‐L −1 compared with the control, 20 and 40 mg NO2 − N‐L −1 . Lipid peroxidation (LPO), as indicated by the thiobarbituric acid reactive substances (TBARS) level, was significantly higher in fish exposed to 330 mg NO2 − N‐L −1 than the control and 20 mg NO2 − N‐L −1 . Moreover, a pro‐oxidant condition was induced when P . orbignyanus were exposed to concentrations from 80 mg NO2 − N‐L −1, which was evidenced by alterations in the antioxidant system and increased levels of LPO. Therefore, it is recommended that the nitrite concentration do not exceed 40 mg NO2 − N‐L −1 in order to avoid oxidative stress for early juvenile Brazilian flounder.