Determination of the Fluoroquinolones Levofloxacin and Ciprofloxacin by a Piezoelectric Immunosensor Modified with Multiwalled Carbon Nanotubes (MWCNTs). (3rd May 2022)

• Record Type: Journal Article
• Authors: Shinko, Evgenia I.; Farafonova, Olga V.; Shanin, Il'ja A.; Eremin, Sergei A.; Ermolaeva, Tatyana N.
• Is Part Of: Analytical letters. Volume 55:Number 7(2022)
• Page Start: 1164
• Page End: 1177
• Publication Date: 2022-05-03
• Subjects: affinity interactions -- ciprofloxacin -- levofloxacin -- multi-walled carbon nanotubes (MWCNTs) -- piezoelectric immunosensor; Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Abstracts; 543
• DOI: 10.1080/00032719.2021.1991364
• Languages: English
• ISSNs: 0003-2719

Abstract

Abstract: Methods for the high-sensitivity determination of fluoroquinolones using a piezoelectric immunosensor based on multi-walled carbon nanotubes (MWCNTs) are developed. The use of MWCNTs in the formation of a stable piezoelectric sensor detection layer increases the active specific surface area which is necessary for receptor molecule binding. The concentrations of hapten-protein conjugates (35/65 serial concentration) and polyclonal antibodies are determined in the direct (15/85 for levofloxacin, 18/82 for ciprofloxacin) and in the competitive immunoassay formats (14/86 for levofloxacin, 10/90 for ciprofloxacin). Conditions for the analysis in the flow-injection mode were studied. The carrier flow rate was 30 and 50 µl/min depending on the detection layer formation method. The characteristics of MWCNTs-based piezoelectric immunosensors were characterized for the determination of fluoroquinolones in the static and in the flow-injection modes. The direct and competitive immunoassay formats (detection limit, linear range of target concentrations, reproducibility) were determined. The detection limits were 9 and 8 ng/ml for levofloxacin and ciprofloxacin in the competitive format and 25 and 21 ng/ml in the direct assay. The piezoelectric immunosensors were employed for the analysis of milk.