Generation of Alveolar Epithelium Using Reconstituted Basement Membrane and hiPSC‐Derived Organoids. Issue 6 (7th January 2022)

• Record Type: Journal Article
• Authors: He, Yong; Rofaani, Elrade; Huang, Xiaochen; Huang, Boxin; Liang, Feng; Wang, Li; Shi, Jian; Peng, Juan; Chen, Yong
• Is Part Of: Advanced healthcare materials. Volume 11:Issue 6(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-01-07
• Subjects: air–blood barriers -- alveolar epithelium -- human‐induced pluripotent stem cell -- organoids -- reconstituted basement membranes; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.202101972
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: In vitro modeling of alveolar epithelium needs to recapitulate features of both cellular and noncellular components of the lung tissues. Herein, a method is presented to generate alveolar epithelium by using human induced pluripotent stem cells (hiPSCs) and reconstituted or artificial basement membrane (ABM). The ABM is obtained by self‐assembling type IV collagen and laminin with a monolayer of crosslinked gelatin nanofibers as backbone and a patterned honeycomb microframe for handling. Alveolar organoids are obtained from hiPSCs and then dissociated into single cells. After replating the alveolar cells on the ABM and a short‐period incubation under submerged and air–liquid interface culture conditions, an alveolar epithelium is achieved, showing high‐level expressions of both alveolar cell‐specific proteins and characteristic tight junctions. Besides, endothelial cells derived from the same hiPSCs are cocultured on the backside of the epithelium, forming an air–blood barrier. The method is generic and can potentially be applied to other types of artificial epithelium and endothelium. Abstract : Artificial basement membrane is obtained by protein self‐assembling in the pore areas of a monolayer of crosslinked nanofibers. Alveolar epithelium is achieved by replating dissociated single cells of alveolar organoids.