3D multiscale analysis of the hierarchical porosity in Coscinodiscus sp. diatoms using a combination of tomographic techniques. Issue 6 (17th February 2022)

• Record Type: Journal Article
• Authors: Darouich, Othmane; Baaziz, Walid; Ihiawakrim, Dris; Hirlimann, Charles; Spehner, Danièle; Schultz, Patrick; Poncet, Hedwige; Rouchon, Virgile; Labidi, Sana; Petit, Corinne; Levitz, Pierre; Ersen, Ovidiu
• Is Part Of: Nanoscale advances. Volume 4:Issue 6(2022)
• Page Start: 1587
• Page End: 1598
• Publication Date: 2022-02-17
• Subjects: 620.5
• DOI: 10.1039/d1na00691f
• Languages: English
• ISSNs: 2516-0230

Abstract

Abstract : A multiscale 3D approach combining three advanced volumetric imaging techniques was employed for obtaining direct information on the hierarchical porosity in Coscinodiscus sp. diatoms having an internal organization ranging from micrometer to nanometer. Abstract : A full 3D analysis of the hierarchical porosity in Coscinodiscus sp. diatom structures was carried out by using a multiscale approach that combines three advanced volumetric imaging techniques with resolutions and fields of view covering all the porous characteristics of such complex architectures: electron tomography, "slice and view" approach that uses a dual-beam microscope (FIB-SEM), and array tomography consisting of serial imaging of ultrathin specimen sections. This multiscale approach allowed the whole porosity network to be quantified and provided an unprecedented structural insight into these natural nanostructured materials with internal organization ranging from micrometer to nanometer. The analysed species is made of several nested layers with different pore sizes, shapes and connectivities and characterized by the presence of interconnected pores structured in various ways. The first evidence of the presence of a nanometric porosity made of ellipsoidal pores in the siliceous diatom frustules is also provided.