Agromyces archimandritae sp. nov., isolated from the cockroach Archimandrita tessellata. Issue 3 (21st March 2022)

• Record Type: Journal Article
• Authors: Guzman, Juan; Ortúzar, Maite; Poehlein, Anja; Daniel, Rolf; Trujillo, Martha E.; Vilcinskas, Andreas
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 3(2022)
• Publication Date: 2022-03-21
• Subjects: Microbacteriaceae -- Agromyces archimandritae -- Archimandrita tesselata -- cockroach microbiota; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005283
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-positive bacterial strain, designated G127AT T, was isolated as soft small white colonies from the hindgut of the cockroach Archimandrita tesselata . Examination of the complete 16S rRNA sequence mapped the strain to the genus Agromyces . The type strain with the highest pairwise similarity was Agromyces marinus H23-8 T (97.3%). The genome of G127AT T was sequenced by a combination of Illumina and Nanopore methods and consisted of a single circular DNA molecule with a size of 3.45 Mb. The DNA G+C content was 71.3 mol%. A phylogenomic tree based on conserved single copy housekeeping genes, placed G127AT T among the ancestral species of the genus Agromyces, and only Agromyces atrinae P27 T was found to diverge earlier than G127AT T . Genome distance metrics average nucleotide identity (ANI) (76–78 %) and digital DNA–DNA hybridization (dDDH) (20.2–21.5 %) of the isolate against available genomes of several type strains of species of the genus Agromyces indicated that G127AT T represented a previously undescribed species of the genus Agromyces . Morphological, physiological and biochemical characteristics, including lipid profile, cellular fatty acids and peptidoglycan type were in accordance with usual attributes of members of the genus Agromyces . The novel isolate could be differentiated from the most closely related species by extracellular expression of acid and alkaline phosphatases, trypsin and α-chymotrypsin, and utilization of l -arabinose and salicin as sole carbon sources. On the basis of the combined genomic and phenotypic features, isolate G127AT T (=DSM 111850 T =LMG 32099 T ) is considered to represent a novel species of the genus Agromyces, for which we propose the name Agromyces archimandritae sp. nov.