Bachu Mushroom Polysaccharide Alleviates Colonic Injury by Modulating the Gut Microbiota. (14th March 2022)

• Record Type: Journal Article
• Authors: Abdureyim, Zumrat; Wang, Lei; Tao, Qing; Xu, Jing; Yimiti, Delixiati; Gao, Qian
• Other Names: Khalaf Osamah Ibrahim Academic Editor.
• Is Part Of: Computational and mathematical methods in medicine. Volume 2022(2022)
• Publication Date: 2022-03-14
• Subjects: Medicine -- Computer simulation -- Periodicals; Medicine -- Mathematical models -- Periodicals; 610.11
• DOI: 10.1155/2022/1353724
• Languages: English
• ISSNs: 1748-670X

Abstract

Abstract : Objective . This study was to define the protective effect of purified Helvella leucopus polysaccharide (p-HLP) against dextran sulfate sodium- (DSS-) induced colitis. Methods . The novel p-HLP was isolated from Bachu mushroom through hot water extraction, ethanol precipitation, and column chromatography. Then, we evaluated the potential effects of p-HLP on colonic histopathology, inflammation, and microbiota composition in DSS-induced colitis mice. Results . p-HLP was a homopolysaccharide with an average molecular weight of 39.14 × 10 8 Da . Functionally, p-HLP significantly attenuated DSS-induced body weight loss and colon shortening. The histological score of the colon lesion was significantly decreased upon p-HLP treatment. Also, p-HLP treatment led to decreased expression of proinflammatory cytokines and mediators (IL-6, IL-1 β and TNF- α, and COX-2 and iNOS) and increased expression of anti-inflammatory cytokine (IL-10) in the colon tissues. Illumina MiSeq sequencing revealed that p-HLP modulated the composition of the gut microbiota. Conclusion . p-HLP is a potent regulator that protects the lesions from DSS-induced colitis.