Super‐resolution fluorescence microscopy‐based single‐molecule spectroscopy. (3rd January 2022)

• Record Type: Journal Article
• Authors: Jeong, Dokyung; Kim, Doory
• Is Part Of: Bulletin of the Korean Chemical Society. Volume 43:Number 3(2022)
• Page Start: 316
• Page End: 327
• Publication Date: 2022-01-03
• Subjects: super‐resolution fluorescence microscopy -- single‐molecule localization microscopy -- single‐molecule spectroscopy -- spectrally resolved super‐resolution microscopy -- single‐molecule sensing; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/bkcs.12471
• Languages: English
• ISSNs: 0253-2964

Abstract

Abstract: The rise of super‐resolution fluorescence microscopy (SRM) has revolutionized our understanding of the nanoscale world. SRM has recently been correlated with spectroscopy techniques to obtain rich spectral information of individual molecules in addition to localization information. This technique ultimately provides multidimensional and functional information of single molecules, which is generally masked in the ensemble averages of previous bulk measurements and creates exciting new opportunities and challenges for single‐molecule spectroscopic imaging. We review the recent developments in single‐molecule spectroscopic imaging technique and how the challenges are addressed to effectively obtain single‐molecule spectroscopic information. Particular attention has been devoted to the applications of this method, including single‐molecule chemical kinetic studies and single‐molecule polarity sensing. Finally, we address the possible future enhancements and applications. Thus, we demonstrate how a single‐molecule spectroscopic imaging technique adds new dimensions of information to the SRM, providing new opportunities in a wide range of research fields. Abstract : Recently developed single‐molecule spectroscopic imaging techniques allow the measurement of the single‐molecule fluorescence spectra in samples with high‐throughput and unveil single‐molecule information that has been masked in ensemble averages. In this review, the applications of these techniques to various areas of chemistry and biology at the single‐molecule level are described, including single‐molecule kinetic study and polarity sensing.