The Rs12569232 SNP Association with Vogt-Koyanagi-Harada Disease and Behcet's Disease is Probably Mediated by Regulation of Linc00467 Expression. (17th November 2021)

• Record Type: Journal Article
• Authors: Wang, Qingfeng; Yi, Shenglan; Du, Ziyu; Huang, Xinyue; Xu, Jing; Cao, Qingfeng; Su, Guannan; Kijlstra, Aize; Yang, Peizeng
• Is Part Of: Ocular immunology and inflammation. Volume 29:Number 7/8(2021)
• Page Start: 1464
• Page End: 1470
• Publication Date: 2021-11-17
• Subjects: Vogt-Koyanagi-Harada disease -- Behcet's disease -- lincRNA -- Cd4+T cells -- single nucleotide polymorphism; Eye -- Diseases -- Immunological aspects -- Periodicals; Eye -- Inflammation -- Periodicals; 617.7
• DOI: 10.1080/09273948.2020.1745244
• Languages: English
• ISSNs: 0927-3948

Abstract

ABSTRACT: Purpose : To investigate whether the rs12569232 SNP association with Vogt-Koyanagi-Harada disease and Behcet's disease is mediated by regulation of Linc00467 expression. Methods : The expression of linc00467 was detected by real-time PCR. Adenovirus carrying the linc00467 was transduced into CD4 + T cells and the effect on cell viability was measured by the CCK-8 test. Human proteome microarray and starBase 2.0 were used to identify the binding proteins of linc00467 and RNA Immunoprecipitation (RIP) was used to confirm the identity of bound proteins. Results : The rs12569232 was associated with the expression of linc00467. The expression of linc00467 was up-regulated in PBMCs and CD4 + T cells from VKH disease and BD patients. Over-expression of linc00467 increased cell viability of CD4 + T cells. HUR was the common binding protein identified by the two methods and confirmed by RIP. Conclusions : The rs12569232 association with VKH disease and BD may be mediated via regulating the expression of linc00467.