Human Hepatocytes Isolated from Explanted Livers: A Powerful Tool to Understand End-stage Liver Disease and Drug Screening. (2nd October 2021)

• Record Type: Journal Article
• Authors: Faccioli, Lanuza A. P.; Kocas-Kilicarslan, Zehra N.; Diaz-Aragon, Ricardo; Motomura, Takashi; Amirneni, Sriram; Malizio, Michelle R.; Coard, Michael C.; Frau, Carla; Haep, Nils; Florentino, Rodrigo M.; Ostrowska, Alina
• Is Part Of: Organogenesis. Volume 17:Number 3/4(2021)
• Page Start: 117
• Page End: 125
• Publication Date: 2021-10-02
• Subjects: Hepatocyte isolation -- cryopreservation -- diseased liver -- drug screening; Morphogenesis -- Periodicals; Morphogenesis; Periodicals; Electronic journals; 571.53
• DOI: 10.1080/15476278.2021.1992216
• Languages: English
• ISSNs: 1547-6278

Abstract

ABSTRACT: The use of primary human hepatocytes has been hampered by limited availability of adequate numbers of fresh and viable cells due to the ongoing shortage of liver donors. Thus, there is no surplus of healthy organs from which freshly isolated cells can be prepared when needed. However, primary hepatocytes can be successfully isolated from explanted liver specimens obtained from patients receiving orthotopic liver transplantation for decompensated liver cirrhosis or for metabolic liver disease without end-stage liver disease and are a valuable resource for the pharmaceutical industry research. This review focuses on the isolation, characterization and cryopreservation of hepatocytes derived from therapeutically resected livers with various hepatic diseases.