MiR-26a-5p suppresses nasopharyngeal carcinoma progression by inhibiting PTGS2 expression. Issue 6 (19th March 2022)

• Record Type: Journal Article
• Authors: Cai, Binlin; Qu, Xiu; Kan, Dan; Luo, Yi
• Is Part Of: Cell cycle. Volume 21:Issue 6(2022)
• Page Start: 618
• Page End: 629
• Publication Date: 2022-03-19
• Subjects: Nasopharyngeal carcinoma -- miR-26a-5p -- PTGS2 -- proliferation -- migration -- invasion; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2022.2030168
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: Nasopharyngeal carcinoma (NPC) has a low five-year survival rate, and its pathogenesis remains unclear. There is an urgent need to improve our understanding of the genetic regulation of NPC tumorigenesis and development. The role of miR-26a-5p in NPC growth regulation and the expression of its target, PTGS2, was analyzed. Quantitative Real-time PCR assay was used to detect miR-26a-5p and PTGS2 expression in human NPC tissues and cell lines. The RNA pull-down dual-luciferase reporter assay was used to determine the association between miR-26a-5p and PTGS2. The effects of miR-26a-5p and PTGS2 on NPC cell viability, proliferation, migration, and invasion were measured by CCK-8, BrdU, and Transwell assays. miR-26a-5p expression in NPC tissues and cell lines was significantly decreased. The overexpression of miR-26a-5p inhibited the viability, proliferation, migration, and invasion of NPC cells. miR-26a-5p bound to the 3-ʹuntranslated region of PTGS2, thus reducing PTGS2 protein levels. miR-26a-5p inhibited NPC development by reducing the expression of its target PTGS2.