Isolation and estimation of flavonoid compound(s) of Baccharoides anthelmintica (L.) Moench. Issue 8 (18th April 2022)

• Record Type: Journal Article
• Authors: Kumar, Deepak; Kaur, Amanpreet; Madaan, Reecha; Kumar, Suresh
• Is Part Of: Natural product research. Volume 36:Issue 8(2022)
• Page Start: 2186
• Page End: 2190
• Publication Date: 2022-04-18
• Subjects: Asteraceae -- Baccharoides anthelmintica -- Butein -- TLC densitometer; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2020.1849204
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Based on ethnobotanical surveys, an Indian traditional plant, i.e., Baccharoides anthelmintica (L.) Moench (Family – Asteraceae) was selected for detailed phytochemical investigations. B. anthelmintica seeds, purchased from local market, were extracted exhaustively in a Soxhlet apparatus using methanol as solvent. A well-established method was adopted to obtain phenolic compounds and flavonoids rich fraction of methanol extract. This fraction yielded two isolates (AK-1 and AK-2) upon purification by column chromatographic process. AK-1 was characterized as mixture of two isomeric compounds − 3′, 4′, 5, 6, 7- and 3′, 4′, 5, 7, 8-pentahydroxy flavanone, and AK-2 as butein (chalcone derivative) by UV, IR and NMR spectral analysis. A TLC densitometric method was developed and validated to estimate the content of butein in B. anthelmintica seeds, and was found to be 1.252 mg/g. Bioactive Marker (butein) based standardized B. anthelmintica seeds will ensure reproducibility in efficacy and safety of the plant or authenticity of its products. Graphical Abstract: