Phytochemical profiling of aqeous methanolic leaf extract of Triclisia gilletii by gas chromatography (GC/MS) and liquid chromatography (HPLC-PDA–ESI/MSn) tandem mass spectroscopy (MS): a pointer to its nephroprotection. Issue 8 (18th April 2022)

• Record Type: Journal Article
• Authors: Olayeriju, Olanrewaju Sam; Papetti, Adele; Colombo, Raffaella; Mannucci, Barbara; Olaleye, Mary Tolulope; Akindahunsi, Akintunde Afolabi
• Is Part Of: Natural product research. Volume 36:Issue 8(2022)
• Page Start: 2171
• Page End: 2176
• Publication Date: 2022-04-18
• Subjects: Triclisia gilletii -- gas chromatography-mass spectrometry -- liquid chromatography-mass spectrometry -- phytochemicals -- nephroprotection; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2020.1845672
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: The phytochemical constituents in the aqueous methanolic leaf extract of Triclisia gilletii responsible for its nephroprotective potentials against ethane-1, 2-diol induced nephrolithiasis as previously investigated in our laboratory were elucidated. The extract was prepared using 80% aqueous methanol in 72 h, Phytochemical contents of aqueous methanolic extract of Triclisia gilletii (TGME) was identified using both a Thermo Scientific DSQII single quadrupole gas chromatography (GC) and a Thermo Scientific liquid chromatography (LCQ Fleet system) tandem mass spectroscopy. The chromatogram acquisition, detection of mass spectral peaks and their waveform processing were performed using Xcalibur MS Software (Thermo Scientific Inc.). GC-MS analysis revealed the presence of phenols, fatty acids, vitamins and steroids. Likewise, for LC-MS analysis kaempferol and dihydrovomifoliol-O-glucoside were detected. The identified constituents have possible contributively effect on the acclaimed pharmacological potential of Triclisia gilletii against ethane-1, 2-diol induced nephrolithiasis. Graphical Abstract: