A simple and rapid method to assay SARS-CoV-2 RNA based on a primer exchange reaction. Issue 28 (18th March 2022)

• Record Type: Journal Article
• Authors: Li, Dayong; Duan, Chengjie; Cheng, Wenting; gong, Youjing; Yao, Yanheng; Wang, Xiaoping; Wang, Zhongyun; Xiang, Yang
• Is Part Of: Chemical communications. Volume 58:Issue 28(2022)
• Page Start: 4484
• Page End: 4487
• Publication Date: 2022-03-18
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d2cc00488g
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Integrating the PER cascade and CRISPR/cas12 system for the rapid detection of SARS-CoV-2 RNA. Abstract : A simple method is proposed in this work for the detection of SARS-CoV-2 RNA based on a primer exchange reaction (PER). By ingeniously integrating the PER cascade and CRISPR/cas12a system, this method can achieve convenient detection of the target RNA in 40 min and distinguish a single-base mutation from the target sequence, demonstrating its superior analytical performance.