Whole blood flow cytometric analysis of Ureaplasma-stimulated monocytes from pregnant women. (June 2015)

• Record Type: Journal Article
• Authors: Friedland, Yael D.; Lee-Pullen, Tracey F.; Nathan, Elizabeth; Watts, Rory; Keelan, Jeffrey A.; Payne, Matthew S.; Ireland, Demelza J.
• Is Part Of: Journal of reproductive immunology. Volume 109(2015)
• Page Start: 84
• Page End: 88
• Publication Date: 2015-06
• Subjects: BFA Brefeldin A -- CCU colour-changing units -- GA gestational age -- IL interleukin -- LPS lipopolysaccharide -- PBMC peripheral blood mononuclear cells -- PRRs pattern recognition receptors -- PTB preterm birth -- spp. species -- TLR Toll-like receptor -- TNF tumour necrosis factor -- U. parvum Ureaplasma parvum -- UpSV3 U. parvum serovar 3; Cytokines -- Monocytes -- Multi-parameter flow cytometry -- Preterm birth -- Ureaplasma spp.; Reproduction -- Immunological aspects -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Reproduction -- Periodicals; Reproduction -- Immunologie -- Périodiques; Immunologie -- Périodiques; Immunology; Reproduction -- Immunological aspects; Periodicals; Electronic journals; Electronic journals; 615.766
• DOI: 10.1016/j.jri.2014.12.008
• Languages: English
• ISSNs: 0165-0378

Abstract

Highlights: Vaginal colonisation with Ureaplasma spp. is common. Ureaplasma spp. are known to cause inflammation-driven preterm birth. Ureaplasma parvum stimulates monocyte cytokine response regardless of maternal colonisation. Ureaplasma spp. colonisation does not tolerise monocytes to subsequent stimulation. Abstract: We hypothesised that circulating monocytes of women with vaginal colonisation with Ureaplasma spp., genital microorganisms known to cause inflammation-driven preterm birth, would elicit a tolerised cytokine response to subsequent in vitro Ureaplasma parvum serovar 3 (UpSV3) stimulation. Using multi-parameter flow cytometry, we found no differences with regard to maternal colonisation status in the frequency of TNF-α-, IL-6-, IL-8- and IL-1β-expressing monocytes in response to subsequent UpSV3 stimulation ( P > 0.10 for all cytokines). We conclude that vaginal Ureaplasma spp. colonisation does not specifically tolerise monocytes of pregnant women towards decreased responses to subsequent stimulation.