Cold tolerance in rice germinating seeds revealed by deep RNAseq analysis of contrasting indica genotypes. (September 2015)

• Record Type: Journal Article
• Authors: Dametto, Andressa; Sperotto, Raul A.; Adamski, Janete M.; Blasi, Édina A.R.; Cargnelutti, Denise; de Oliveira, Luiz F.V.; Ricachenevsky, Felipe K.; Fregonezi, Jeferson N.; Mariath, Jorge E.A.; da Cruz, Renata P.; Margis, Rogério; Fett, Janette P.
• Is Part Of: Plant science. Volume 238(2015:Sep.)
• Page Start: 1
• Page End: 12
• Publication Date: 2015-09
• Subjects: OsEXD Expressed protein -- OsCYC Cyclin -- OsCDKB2 ;1 Cyclin-dependent kinase B2-1 -- OsCSLE1 Cellulose synthase-like family E -- OsAQU Aquaporin protein -- OsKET 3-ketoacyl-CoA synthase -- OsPRX Peroxidase precursor -- OsFBX221 F-box domain-containing protein -- OsAUX OsIAA13 – Auxin-responsive Aux/IAA gene family member -- OsDHN Dehydrin -- OsLEA Late embryogenesis abundant protein -- OsUBQ5 Ubiquitin 5; Cell division -- Cold tolerance -- Germination -- Indica rice -- Seed imbibition -- Stress response; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2015.05.009
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Cold-regulated genes were identified by RNAseq in rice germinating seeds. Cold-tolerant (CT) genotype showed 1, 361 genes differentially regulated by cold. Cell division and expansion processes are more active in CT genotype. Fatty acid unsaturation and antioxidant capacity are more active in CT genotype. Abstract: Rice productivity is largely affected by low temperature, which can be harmful throughout plant development, from germination to grain filling. Germination of indica rice cultivars under cold is slow and not uniform, resulting in irregular emergence and small plant population. To identify and characterize novel genes involved in cold tolerance during the germination stage, two indica rice genotypes (sister lines previously identified as cold-tolerant and cold-sensitive) were used in parallel transcriptomic analysis (RNAseq) under cold treatment (seeds germinating at 13 °C for 7 days). We detected 1, 361 differentially expressed transcripts. Differences in gene expression found by RNAseq were confirmed for 11 selected genes using RT-qPCR. Biological processes enhanced in the cold-tolerant seedlings include: cell division and expansion (confirmed by anatomical sections of germinating seeds), cell wall integrity and extensibility, water uptake and membrane transport capacity, sucrose synthesis, generation of simple sugars, unsaturation of membrane fatty acids, wax biosynthesis, antioxidant capacity (confirmed by histochemical staining of H2 O2 ), and hormone and Ca 2+ -signaling. The cold-sensitive seedlings respond to low temperature stress increasing synthesis of HSPs and dehydrins, along with enhanced ubiquitin/proteasome protein degradation pathway and polyamine biosynthesis. Our findings can be useful in future biotechnological approaches aiming to cold tolerance in indica rice.