The effect of manganese-induced toxicity on the cytokine mRNA expression of chicken spleen lymphocytes in vitro. (August 2015)

• Record Type: Journal Article
• Authors: Lu, Xinxing; Zhu, Yihao; Bai, Rensheng; Li, Shu; Teng, Xiaohua
• Is Part Of: Research in veterinary science. Volume 101(2015)
• Page Start: 165
• Page End: 167
• Publication Date: 2015-08
• Subjects: Manganese -- Chicken -- Spleen -- Lymphocyte -- Cytokine; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2015.05.009
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: Manganese (Mn) is essential for life, but excess Mn exposure is harmful. This study investigated the effect of excess Mn on the cytokines of spleen lymphocytes in chicken. Lymphocytes were incubated with or without MnCl2 (2, 4, 6, and 8 × 10 − 4 mmol/L) for 12, 24, 36, and 48 h, respectively. The mRNA expression of interleukin (IL) -2, IL-4, IL-6, IL-12β, and IL-17 and interferon (INF) -γ was examined using RT-PCR. Excess Mn inhibited IL-2, IL-4, IL-6, IL-12β, and IL-17 mRNA expression in chicken spleen lymphocytes in a dose-dependent manner. IFN-γ was inhibited by 8 × 10 − 4 mmol/L Mn for 48 h. This study demonstrates that excess Mn affects cytokine mRNA expression and causes immunosuppression in chicken spleen lymphocytes. The relationships between IL-6 and IL-17 and between IL-2 and IL-12β were strong under immunosuppression caused by excess Mn in lymphocytes. Highlights: Excess Mn inhibits mRNA expression of IL-2, IL-4, IL-6, IL-12β, and IL-17. Mn has a dose-dependent effect on IL-2, IL-4, IL-6, IL-12β, and IL-17. The relationships between IL-2 and IL-12β and between IL-6 and IL-17 are strong. Excess Mn causes immunosuppression in chicken spleen lymphocytes.