Serum concentrations of canine interleukin-1 receptor antagonist protein in healthy dogs after incubation using an autologous serum processing system. (August 2015)

• Record Type: Journal Article
• Authors: Huggins, S.S.; Suchodolski, J.S.; Bearden, R.N.; Steiner, J.M.; Saunders, W.B.
• Is Part Of: Research in veterinary science. Volume 101(2015)
• Page Start: 28
• Page End: 33
• Publication Date: 2015-08
• Subjects: Autologous serum -- Canine interleukin-1 receptor antagonist -- ELISA -- Orthobiologics -- Osteoarthritis; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2015.05.012
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: The objectives of this study were to optimize and validate a canine IL-1RA ELISA using commercially available reagents and to determine the effect of an autologous serum processing system (IRAP II) on IL-1RA concentrations in canine serum. The clinical detection limit of the optimized ELISA was 188.8 to 39, 965.6 pg/mL. The observed-to-expected ratio (O:E) for three serial dilutions for four serum samples ranged from 109.6 to 132.2%. The O:E for four serum samples spiked with four concentrations of canine IL-1 RA ranged from 98.7 to 114.3%. Coefficients of variances for intra- and interassay variability ranged from 1.4 to 3.0 and 6.3 to 9.8, respectively. The ELISA was sensitive, linear, accurate, precise, and reproducible. Mean ± SD serum concentration of IL-1RA in 12 healthy dogs was 396.6 ± 208.0 pg/mL. There was a significant increase in IL-1RA when blood was incubated in the IRAP II system (15, 955.0 ± 6421.0 pg/mL, P < 0.0001). Highlights: Autologous serum systems that enrich IL-1RA may prove useful for treating canine osteoarthritis. A linear, accurate, precise, and repeatable canine IL-1RA ELISA has yet to be described. We report the optimization and validation of a canine IL-1RA ELISA for use on canine serum. Using this ELISA, IL-1 RA levels in healthy canine blood are 396.6 ± 208.0 pg/mL (mean ± SD). IL-1 levels increased to 15, 955.0 ± 6421.0 pg/mL after treatment with an autologous serum system.