Expression of inflammation-associated genes in circulating leukocytes and activity of indoleamine-2, 3-dioxygenase in dairy cattle with acute puerperal metritis and bacteremia. (August 2015)

• Record Type: Journal Article
• Authors: Credille, Brent C.; Woolums, Amelia R.; Overton, Michael W.; Hurley, David J.; Giguère, Steeve
• Is Part Of: Research in veterinary science. Volume 101(2015)
• Page Start: 6
• Page End: 10
• Publication Date: 2015-08
• Subjects: APM acute puerperal metritis -- IL interleukin -- IDO indoleamine-2, 3-dioxygenase -- PBMC peripheral blood mononuclear cell -- LPS lipopolysaccharide -- HPLC high performance liquid chromatography -- DIM days in milk -- qRT-PCR quantitative reverse transcriptase polymerase chain reaction; Uterine -- Blood culture -- Cytokine -- Metritis -- Dairy -- Polymerase chain reaction; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2015.05.013
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: Objective: To investigate whether expression of genes associated with inflammation and activity of indoleamine-2, 3-dioxygenase (IDO) correlated with disease status and prevalence of bacteremia in post-partum dairy cattle with and without acute puerperal metritis (APM). Procedures: Blood was collected from cattle with APM and control cattle matched by parity and days in milk. Leukocytes were isolated and expression of 6 genes was quantified. Activity of IDO was measured in serum with higher performance liquid chromatography (HPLC). Results: The relative expression of IL-1β in cattle with APM was significantly lower than that in controls. IDO activity was not significantly different between bacteremic and non-bacteremic cattle Conclusions and clinical relevance: The expression of IL-1β was lower in cattle with APM. The lower levels of IL-1β expression in PBMCs of cattle with APM suggest impaired inflammatory responses and may contribute to the development of the disease in this population of animals. Highlights: We examine the expression of inflammatory cytokine genes in dairy cattle with metritis and bacteremia. We examine the activity of indoleamine-2, 3-dioxygenase (IDO) in dairy cattle with metritis and bacteremia. Cattle with metritis have decreased levels of IL-1 expression compared to healthy controls. Bacteremic cattle have a tendency towards higher levels of expression of both IL-4 and IL-6. No difference in the activity of IDO was found between groups.