MiR-9-1 Suppresses Cell Proliferation and Promotes Apoptosis by Targeting UHRF1 in Lung Cancer. (September 2021)

• Record Type: Journal Article
• Authors: Jia, Cheng-You; Xiang, Wei; Liu, Ji-Bin; Jiang, Geng-Xi; Sun, Feng; Wu, Jian-Jun; Yang, Xiao-Li; Xin, Rui; Shi, Yi; Zhang, Dan-Dan; Li, Wen; Zuberi, Zavuga; Zhang, Jie; Lu, Gai-Xia; Wang, Hui-Min; Wang, Pei-Yao; Yu, Fei; Lv, Zhong-Wei; Ma, Yu-Shui; Fu, Da
• Is Part Of: Technology in cancer research & treatment. Volume 20(2021)
• Publication Date: 2021-09
• Subjects: miR-9 to 1 -- UHRF1 -- lung cancer -- proliferation -- apoptosis; Oncology -- Periodicals; Cancer -- Diagnosis -- Periodicals; Cancer -- Treatment -- Technological innovations -- Periodicals; 616.994
• DOI: 10.1177/15330338211041191
• Languages: English
• ISSNs: 1533-0346

Abstract

Lung cancer is listed as the most common reason for cancer-related death all over the world despite diagnostic improvements and the development of chemotherapy and targeted therapies. MicroRNAs control both physiological and pathological processes including development and cancer. A microRNA-9 to 1 (miR-9 to 1) overexpression model in lung cancer cell lines was established and miR-9 to 1 was found to significantly suppress the proliferation rate in lung cancer cell lines, colony formation in vitro, and tumorigenicity in nude mice of A549 cells. Ubiquitin-like containing PHD and RING finger domains 1 (UHRF1) was then identified to direct target of miR-9 to 1. The inhibition of UHRF1 by miR-9 to 1 causes G1 arrest and p15, p16, and p21 were re-expressed in miR-9 to 1 group in mRNA level and protein level. Silence of UHRF1 expression in A549 cells resulted in the similar re-expression of p15, p16, p21 which is similar with miR-9 to 1 infection. Therefore, we concluded that UHRF1 is a new target for miR-9 to 1 to suppress cell proliferation by re-expression of tumor suppressors p15, p16, and p21 mediated by UHRF1.