Photo‐Degradable Protein‐Polymer Hybrid Shells for Caging Living Cells. Issue 12 (3rd February 2022)

• Record Type: Journal Article
• Authors: Yamaguchi, Satoshi; Chujo, Kazuki; Ohashi, Noriyuki; Minamihata, Kosuke; Nagamune, Teruyuki
• Is Part Of: Chemistry. Volume 28:Issue 12(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-02-03
• Subjects: bioorganic chemistry -- cage compounds -- cell adhesion -- layer-by-layer assembly -- protein-polymer hybrid materials; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.202103941
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract: There is growing demand for the precise remote control of cellular functions in various fields. Herein, a method for caging mammalian cells by coating with photodegradable protein‐polymer hybrid shells to photo‐control their functions without genetic engineering is reported. A layer‐by‐layer assembly of photocleavable synthetic materials through biotin‐streptavidin (SA) binding was employed for cell coating. The cell surfaces were first biotinylated with photocleavable biotinylated poly(ethylene glycol)(PEG)‐lipid and then coated by repeatedly layering SA and micelles of the PEG‐lipid and photocleavable biotinylated four‐arm PEG. The cell extension and adhesion were suppressed with the shells and then triggered with the degradation of the shells by light exposure. Macrophage phagocytosis was also stopped by caging with the shells and restarted by light‐guided uncaging. This study provides the first proof of principle that cellular functions can be remotely controlled by steric hinderance of cell surfaces with photodegradable materials. Abstract : A new strategy towards the remote control of cell functions is reported. Photo‐degradable protein‐polymer hybrid shells for protecting the whole cell surface were developed by the layer‐by‐layer assembly of polymers and polymer micelles through protein‐ligand binding. These shells can be used to photo‐regulate cellular functions by steric hinderance against the interaction between cell surfaces and substrates.