Effect of inactivated nature‐derived microbial composition on mouse immune system. Issue 3 (6th December 2021)

• Record Type: Journal Article
• Authors: González‐Rodríguez, Martín Ignacio; Nurminen, Noora; Kummola, Laura; Laitinen, Olli H.; Oikarinen, Sami; Parajuli, Anirudra; Salomaa, Tanja; Mäkelä, Iida; Roslund, Marja I.; Sinkkonen, Aki; Hyöty, Heikki; Junttila, Ilkka S.
• Is Part Of: Immunity, inflammation and disease. Volume 10:Issue 3(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-12-06
• Subjects: rodent -- animals -- autoimmunity -- diseases -- T cells -- cells; Immunology -- Periodicals; Immunity -- Periodicals; Inflammation -- Periodicals; 616.079
• DOI: 10.1002/iid3.579
• Languages: English
• ISSNs: 2050-4527

Abstract

Abstract: Introduction: The hygiene hypothesis suggests that decrease in early life infections due to increased societal‐level hygiene standards subjects one to allergic and autoimmune diseases. In this report, we have studied the effect of sterilized forest soil and plant‐based material on mouse immune system and gut microbiome. Methods: Inbred C57Bl/6 mice maintained in normal sterile environment were subjected to autoclaved forest soil‐derived powder in their bedding for 1 h a day for 3 weeks. Immune response was measured by immune cell flow cytometry, serum cytokine enzyme‐linked immunoassay (ELISA) and quantitative polymerase chain reaction (qPCR) analysis. Furthermore, the mouse gut microbiome was analyzed by sequencing. Results: When compared to control mice, mice treated with soil‐derived powder had decreased level of pro‐inflammatory cytokines namely interleukin (IL)−17F and IL‐21 in the serum. Furthermore, splenocytes from mice treated with soil‐derived powder expressed less IL‐1b, IL‐5, IL‐6, IL‐13, and tumor necrosis factor (TNF) upon cell activation. Gut microbiome appeared to be stabilized by the treatment. Conclusions: These results provide insights on the effect of biodiversity on murine immune system in sterile environment. Subjecting mice to soil‐based plant and microbe structures appears to elicit immune response that could be beneficial, for example, in type 2 inflammation‐related diseases, that is, allergic diseases. Abstract : Autoclaved forest soil extract was added to the bedding of standard laboratory mice. The immune response of mice was measured indicating decreased pro‐inflammatory cytokine production in soil‐subjected mice.