Dose‐dependent effect of myelin oligodendrocyte glycoprotein on visual function and optic nerve damage in experimental autoimmune encephalomyelitis. Issue 3 (18th January 2022)

• Record Type: Journal Article
• Authors: Castoldi, Valerio; Marenna, Silvia; Huang, Su‐Chun; d'Isa, Raffaele; Chaabane, Linda; Comi, Giancarlo; Leocani, Letizia
• Other Names: Marusak Hilary guestEditor.
• Is Part Of: Journal of neuroscience research. Volume 100:Issue 3(2022)
• Page Start: 855
• Page End: 868
• Publication Date: 2022-01-18
• Subjects: experimental autoimmune encephalomyelitis -- myelin oligodendrocyte glycoprotein -- optic neuritis -- RRID:AB_1158338 -- RRID:AB_2566782 -- RRID:AB_305055 -- RRID:AB_839504 -- RRID:SCR_019096 -- visual evoked potentials; Neurobiology -- Periodicals; 612
• DOI: 10.1002/jnr.25007
• Languages: English
• ISSNs: 0360-4012

Abstract

Abstract: Female Dark Agouti rats were immunized with increasing doses of myelin oligodendrocyte glycoprotein (MOG) to develop experimental autoimmune encephalomyelitis (EAE), a preclinical model of multiple sclerosis. Typical EAE motor impairments were assessed daily and noninvasive visual evoked potentials (VEPs) were recorded at baseline and 5 weeks after immunization, with final histopathology of optic nerves (ONs). Immunized rats exhibited a relapsing‐remitting clinical course. Both VEP and histological abnormalities were detected in a MOG dose‐dependent gradient. Increasing MOG dosage augmented visual function impairment in EAE, which could be monitored with VEP recording to assess demyelination and axonal loss along ONs. Abstract : In rats with experimental autoimmune encephalomyelitis, increasing myelin oligodendrocyte glycoprotein (MOG) quantity produced optic nerve (ON) damage in a dose‐dependent gradient. Visual function was measured through visual evoked potential (VEP), with final ON histology. Forty and fifty micrograms of MOG resulted in VEP latency increase, correlating with demyelination, whereas 60 µg caused VEP amplitude decrease associated with axonal loss.