Metallothionein-1 gene from Exopalaemon carinicauda and its response to heavy metal ions challenge. (February 2022)

• Record Type: Journal Article
• Authors: Liu, Yujie; Wu, Zixuan; Guo, Kun; Zhou, Yongzhao; Xing, Kefan; Zheng, Jiaqi; Sun, Yuying; Zhang, Jiquan
• Is Part Of: Marine pollution bulletin. Volume 175(2022)
• Publication Date: 2022-02
• Subjects: Metallothionein -- Crustacean -- Copper -- Cadmium -- Developmental stage; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2022.113324
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: Metallothioneins (MTs) belong to a conserved low-molecular-weight protein family that participates in heavy metal binding and detoxification. EcMT-1 was amplified by PCR from genomic DNA of Exopalaenon carinicauda . It contained a 180 bp open reading frame and encoded 59 amino acids. A total of 18 cysteine (Cys) residues were found in the deduced amino acid sequence, which was consistent with the Cys-rich characteristics of MTs. EcMT-1 was mainly expressed in hepatopancreas, followed by stomach and gill. The expression profiles of EcMT-1 indicated that EcMT-1 was significantly increased at 24, 48 h and 12, 24, and 48 h under the treatment of 2.5 μmol/L CdCl2 and 50 μmol/L CuSO4 . The expression of EcMT-1 at gastrula stage was very low; it was detectable until nauplius stage, and the highest expression level appeared in the postlarvae stage. Graphical abstract: Unlabelled Image Highlights: EcMT-1 gene was obtained from E. carinicauda . EcMT-1 was predominantly expressed in hepatopancreas of E. carinicauda . EcMT-1 was up-regulated after challenge with CdCl2 or CuSO4 . EcMT-1 was detectable at nauplius stage.