Myo-inositol improves growth performance and regulates lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis) fed different percentage of lipid. Issue 5 (14th March 2022)

• Record Type: Journal Article
• Authors: Bu, Xianyong; Wang, Xiaodan; Lin, Zhideng; Wang, Chunling; Li, Lingyu; Liu, Shubin; Shi, Qingchao; Qin, Jian G.; Chen, Liqiao
• Is Part Of: British journal of nutrition. Volume 127:Issue 5(2022)
• Page Start: 666
• Page End: 678
• Publication Date: 2022-03-14
• Subjects: Myo-inositol -- Eriocheir sinensis -- Lipid metabolism -- Regulatory pathways -- High lipid diet; Nutrition -- Periodicals; 572.4
• DOI: 10.1017/S0007114521001409
• Languages: English
• ISSNs: 0007-1145

Abstract

Abstract: This study evaluated the effects of dietary myo -inositol (MI) on growth performance, antioxidant status and lipid metabolism of juvenile Chinese mitten crab ( Eriocheir sinensis ) fed different percentage of lipid. Crabs (4·58 (sem 0·05) g) were fed four diets including a normal lipid diet (N, containing 7 % lipid and 0 mg/kg MI), N with MI supplementation (N + MI, containing 7 % lipid and 1600 mg/kg MI), a high lipid diet (H, containing 13 % lipid and 0 mg/kg MI) and H with MI supplementation (H + MI, containing 13 % lipid and 1600 mg/kg MI) for 8 weeks. The H + MI group showed higher weight gain and specific growth rate than those in the H group. The dietary MI could improve the lipid accumulations in the whole body, hepatopancreas and muscle as a result of feeding on the high dietary lipid (13 %) in crabs. Besides, the crabs fed the H + MI diets increased the activities of antioxidant enzymes but reduced the malondialdehyde content in hepatopancreas compared with those fed the H diets. Moreover, dietary MI enhanced the expression of genes involved in lipid oxidation and exportation, yet reduced lipid absorption and synthesis genes expression in the hepatopancreas of crabs fed the H diet, which might be related to the activation of inositol 1, 4, 5-trisphosphate receptor (IP3R)/calmodulin-dependent protein kinase kinase- β (CaMKK β )/adenosine 5'-monophosphate-activated protein kinase (AMPK) signalling pathway. This study demonstrates that MI could increase lipid utilisation and reduce lipid deposition in the hepatopancreas of E. sinensis fed a high lipid diet through IP3R/CaMKK β /AMPK activation. This work provides new insights into the function of MI in the diet of crustaceans.