Cytomegalovirus Infection: Mouse Model. (25th July 2018)

• Record Type: Journal Article
• Authors: Brizić, Ilija; Lisnić, Berislav; Brune, Wolfram; Hengel, Hartmut; Jonjić, Stipan
• Editors: Coligan, John E.; Bierer, Barbara E.; Margulies, David H.; Shevach, Ethan M.; Strober, Warren
• Is Part Of: Current protocols in immunology. Volume 122(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-07-25
• Subjects: cytomegalovirus infections -- disease models -- fibroblasts -- herpesviridae infections -- MCMV -- mice -- mouse cytomegalovirus -- salivary gland -- viral load -- viral plaque assay -- virology -- virus replication; Immunology -- Laboratory manuals; Medical protocols; Immunologic Techniques; Medical protocols; Immunology; Laboratory manuals; Reference works; 571.96028
• DOI: 10.1002/cpim.51
• Languages: English
• ISSNs: 1934-3671

Abstract

Abstract: This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (MCMV). Methods are included for propagating MCMV in cell cultures and for preparing a more virulent form of MCMV from salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, a method is described for preparing the murine embryonic fibroblasts used for propagating MCMV and for the plaque assay. © 2018 by John Wiley & Sons, Inc.