A New Lipid‐Droplets‐Targeted Fluorescence Probe with Dual‐Reactive Sites for Specific Detection of ClO− in Living Cells. Issue 7 (17th February 2022)

• Record Type: Journal Article
• Authors: Li, Zhao; Yang, Yuan; Yin, Pei; Yang, Zengming; Zhang, Bo; Zhang, Shengjun; Han, Bingyang; Lv, Jiawei; Dong, Fenghao; Ma, Hengchang
• Is Part Of: ChemistrySelect. Volume 7:Issue 7(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-02-17
• Subjects: Lipid droplets -- aggregation-induced-emission (AIE) -- ClO− detection -- cell imaging; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.202104525
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: Lipid droplets (LDs) are organelles composed of a lipid core surrounded by a phospholipid monolayer, plays an important role in a variety of physiological processes as well as diseases. In this work, TPAs‐SCH3 ‐2CN with an enhanced electron D‐π‐A system, is synthesized, which makes itself as an orange emission compound (Em =590 nm) and also have high polarity. It is verified that TPAs‐SCH3 ‐2CN is an excellent LDs‐targeted probe, and it also sensitive to the ClO − detection in vitro and in cells. Impressively, due to the high lipophilicity, TPAs‐SCH3 ‐2CN is able to stain the yolk lipids in zebrafish, exhibiting the potential for monitoring lipid transport and metabolism processes. Abstract : A fluorescent AIEgen of TPAs‐SCH3‐2CN with an enhanced electron D‐π‐A system was synthesized, and developed as a versatile probe to lipid droplets (LDs)‐targeted imaging and the ROS species of ClO ‐ detection in vitro and in cells. Due to the accumulation into the yolk lipids in zebrafish, TPAs‐SCH3‐2CN was verified with the great potential for monitoring lipid transport and metabolism processes in zebrafish.