A comparative study of chiral separation of proton pump inhibitors by supercritical fluid chromatography and high‐performance liquid chromatography. Issue 4 (19th December 2021)

• Record Type: Journal Article
• Authors: Raja, Rupak; Alam, Syed Dilshad; Srisath, Vikas; Jain, Arvind Kumar; ALOthman, Zeid A.; Mohammed, Abdallah A. A.; Islam, Mohammad Ataul; Bhatt, Tahir; Ali, Imran
• Is Part Of: Journal of separation science. Volume 45:Issue 4(2022)
• Page Start: 804
• Page End: 811
• Publication Date: 2021-12-19
• Subjects: chiral separations -- high‐performance liquid chromatography -- proton pump inhibitors -- supercritical fluid chromatography; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.202100834
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract: A comparative study of chiral separation of pantoprazole and rabeprazole is carried out using supercritical fluid chromatography and high‐performance liquid chromatography. The columns used were Chiralpak IA and Chiralpak IE. The best mobile phase in supercritical fluid chromatography was carbon dioxide‐0.2% triethylamine in methanol (60:40) and 0.1% triethylamine in n ‐hexane‐ethanol (50:50) in high‐performance liquid chromatography. For supercritical fluid chromatography, values of the retention factor of pantoprazole enantiomers were 3.97 and 4.88. These values for rabeprazole enantiomers were 6.10 and 7.52. The values of separation and resolution factor for pantoprazole and rabeprazole were 1.23 and 1.23 and 2.20 and 3.36, respectively. Similarly, for high‐performance liquid chromatography, the values of retention factor for enantiomers of pantoprazole were 4.02 and 7.32. These values for rabeprazole enantiomers were 5.32 and 7.88, respectively. The values of separation and resolution factor for pantoprazole and rabeprazole were 1.82 and 1.48 and 9.22 and 6.58, respectively. A comparison was carried out, which confirmed supercritical fluid chromatography as the best method due to its fastness, eco‐friendly, and inexpensiveness. The reported methods are effective, efficient, and reproducible and may be used to separate and identify pantoprazole and rabeprazole in any unknown samples.