Cytogenotoxic evaluation of the acetonitrile extract, citrinin and dicitrinin-A from Penicillium citrinum. (4th March 2022)

• Record Type: Journal Article
• Authors: de Oliveira Filho, José Williams Gomes; Andrade, Teresinha de Jesus Aguiar dos Santos; de Lima, Rosália Maria Tôrres; Silva, Dulce Helena Siqueira; dos Reis, Antonielly Campinho; Santos, José Victor de Oliveira; de Meneses, Ag-Anne Pereira Melo; de Carvalho, Ricardo Melo; da Mata, Ana Maria Oliveira; de Alencar, Marcus Vinícius Oliveira Barros; Dias, Ana Carolina Soares; da Silva, Felipe Cavalcanti Carneiro; Islam, Muhammad Torequl; Clark, Cain C. T.; Sousa, João Marcelo de Castro e; Melo-Cavalcante, Ana Amélia de Carvalho
• Is Part Of: Drug and chemical toxicology. Volume 45:Number 2(2022)
• Page Start: 688
• Page End: 697
• Publication Date: 2022-03-04
• Subjects: Endophytic fungi -- Penicillium citrinum -- mycotoxins -- cytotoxicity -- genotoxicity -- mutagenicity; Toxicology -- Periodicals; Drugs -- Toxicology -- Periodicals; Toxicology, Experimental -- Periodicals; 615.9005
• DOI: 10.1080/01480545.2020.1769642
• Languages: English
• ISSNs: 0148-0545

Abstract

Abstract: Endophytic fungi are promising sources of bioactive substances; however, their secondary metabolites are toxic to plants, animals, and humans. This study aimed toevaluate the toxic, cytotoxic, mutagenic and oxidant/antioxidant activities of acetonitrile extract (AEPc), citrinin (CIT) and dicitrinin-A (DIC-A) of Penicillium citrinum . For this, the test substances at 0.5; 1.0; 1.5 and 2 μg/mLwere exposed for 24 and 48 h in Artemia salina, and 48 h in Allium cepa test systems. The oxidant/antioxidant test was evaluated in pre-, co- and post-treatment with the stressor hydrogen peroxide (H2 O2 ) in Saccharomyces cerevisiae . The results suggest that the AEPc, CIT and DIC-A at 0.5; 1.0; 1.5 and 2 μg/mL showed toxicity in A. saline, with LC50 (24 h) of 2.03 μg/mL, 1.71 μg/mL and 2.29 μg/mL, and LC50 (48 h) of 0.51 μg/mL, 0.54 μg/mL and 0.54 μg/mL, respectively.In A. cepa, the test substances also exerted cytotoxic and mutagenic effects. The AEPc, CIT and DIC-A at lower concentrations modulated the damage induced by H2 O2 in the proficient and mutant strains of S. cerevisiae for cytoplasmic and mitochondrial superoxide dismutase. Moreover, the AEPc at 2 μg/mL and CIT at the two highest concentrations did not affect the H2 O2 -induced DNA damage in the test strains. In conclusion, AEPc, CIT and DIC-A of P. citrinum may exert their toxic, cytotoxic and mutagenic effects in the test systems possibly through oxidative stress induction pathway.