E0224 Model of cardiac arrest in rats by transcutaneous electrical epicardium stimulation. (17th November 2010)

• Record Type: Journal Article
• Authors: Ji-yan, Lin; Xin, Li; Hui, Li; Hong-yan, Wei; Rong, Liu; Chun-lin, Hu; Gang, Dai; Xiao-xing, Liao
• Is Part Of: Heart. Volume 96(2010)Supplement 3
• Page Start: A72
• Page End: A72
• Publication Date: 2010-11-17
• Subjects: Cardiac arrest -- cardiopulmonary resuscitation -- epicardium -- electrical stimulation -- animal mode; Heart -- Diseases -- Treatment -- Periodicals; Cardiology -- Periodicals; 616.12
• DOI: 10.1136/hrt.2010.208967.224
• Languages: English
• ISSNs: 1355-6037

Abstract

Abstract : Objective: To establish a new model of Cardiac Arrest (CA) in rats by transcutaneous electrical epicardium stimulation. Methods: Two acupuncture needles connected to the anode and cathode of a stimulator were transcutaneously inserted into the epicardium as electrodes. The stimulating current was steered to the epicardium and the stimulation was maintained for 3 min to induce CA. Cardiopulmonary resuscitation (CPR) was performed at 6 min after a period of nonintervention. Results: The success rate of induction was 12/20 at the current intensity of 1 mA; and reached 20/20 when the current intensity was increased to 2 mA. The average time from the electrical stimulation to CA induction was 5.10 (±2.81) s. When the electrical stimulation stopped, 18/20 rats had ventricular fibrillation and 2/20 rats had pulseless electrical activity. CPR was performed for averagely 207.4 (±148.8) s. The restoration of spontaneous circulation was 20/20. The death rate within 4 h after CA was 5/20, and the 72-h survival rate was 10/20. There were only two cases of complications, a minor muscle contraction and a minor lung lobe injury. Conclusion: The model of CA in rats induced by transcutaneous electrical epicardium stimulation is a stable model that requires low-intensity current and has fewer complications.