A DNase-mimetic artificial enzyme for the eradication of drug-resistant bacterial biofilm infections. Issue 7 (2nd February 2022)

• Record Type: Journal Article
• Authors: Hu, Haolu; Kang, Xiaoxia; Shan, Zhenhua; Yang, Xiaoxiao; Bing, Wei; Wu, Li; Ge, Haiyan; Ji, Haiwei
• Is Part Of: Nanoscale. Volume 14:Issue 7(2022)
• Page Start: 2676
• Page End: 2685
• Publication Date: 2022-02-02
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/d1nr07629a
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : GO-NTA-Ce has DNase-like enzyme properties and excellent photothermal effect. The combination of photothermal treatment with chemical degradation and physical destruction can completely kill bacteria encased in biofilms. Abstract : The construction of multifunctional nano-enzymes is a feasible strategy for fighting multi-drug resistant (MDR) bacterial biofilm-associated infections. Extracellular DNA (eDNA) is an important functional part of biofilm formation, including the initial adherence of bacteria to subsequent development and eventual maturation. A nano-enzyme platform of graphene oxide-based nitrilotriacetic acid-cerium(iv ) composite (GO-NTA-Ce) against bacterial biofilm infection has been developed. When located at the site of bacteria-associated infection, GO-NTA-Ce could inhibit the biofilm formation and effectively disperse the formed biofilm by degrading the eDNA. In addition to Ce-mediated deoxyribonuclease (DNase)-like activity, near-infrared laser irradiation of GO-NTA-Ce could produce local hyperthermia to kill the bacteria that lost the protection by the biofilm matrix. In addition, graphene is also a new green broad-spectrum antimicrobial material that can exert its antimicrobial effects through physical damage and chemical damage. In short, our GO-NTA-Ce nano-enzyme platform is capable of effectively eradicating drug-resistant bacterial biofilm infections through the triple action of DNase-like enzyme properties, photothermal therapy, and graphene-based antimicrobial activity, and the nano-composite has excellent potential for the treatment of MDR bacterial biofilm infections.