Circular RNA circRNA_0067934 promotes glioma development by modulating the microRNA miR-7/ Wnt/β-catenin axis. Issue 3 (1st March 2022)

• Record Type: Journal Article
• Authors: Pei, Yunlong; Zhang, Hongying; Lu, Kongye; Tang, Xiaojia; Li, Jialing; Zhang, Enpeng; Zhang, Jun; Huang, Yujia; Yang, Zhijie; Lu, Zhenggang; Li, Yuping; Zhang, Hengzhu; Dong, Lun
• Is Part Of: Bioengineered. Volume 13:Issue 3(2022)
• Page Start: 5792
• Page End: 5802
• Publication Date: 2022-03-01
• Subjects: circRNA_0067934 -- circRNAs -- miR-7 -- glioma; Biomedical engineering -- Periodicals; Biotechnology -- Periodicals; Microbiology -- Periodicals; 660.6
• DOI: 10.1080/21655979.2022.2033382
• Languages: English
• ISSNs: 2165-5987

Abstract

ABSTRACT: Glioma, one of the most prevalent malignant tumors, is well-known for its poor prognosis and low survival rate among patients. As a type of non-coding RNA, circular RNAs (circRNAs) play a significant role in tumor progression. However, the function and role of circRNAs in glioma development remain unclarified. In our experiments, the relative expression level of circRNA_0067934 and miR-7 in glioma tissue was detected by qRT-PCR, and specific gene knockdown was mediated by siRNA and miRNA-inhibitor. Dual-luciferase reporter assay was carried out to determine whether miR-7 successfully targeted circRNA_0067934. Also, CCK-8 and Transwell were performed to evaluate the malignant behaviors of glioma tissues. Western blotting and immunofluorescence were used to evaluate relative protein expression levels. The results of qRT-PCR indicated that circRNA_0067934 was over-expressed in glioma tissues, and down regulation of circRNA_0067934 reduced the tumor progression by inhibiting cell proliferation, invasion, and migration. The relative expression level of miR-7 was significantly reduced in glioma tissues, which showed a negative association with the expression of circRNA_0067934. CircRNA_0067934 could tagete the miR-7 to regulate progression of glioma cell. In addition, the Wnt/β-catenin signaling pathway might involve in down stream regulation of circRNA_0067934 and miR-7. In conclusion, our results revealed that circRNA_0067934 regulates glioma cells progression by targeting miR-7/ Wnt/β-catenin axis. Graphical Abstract: uf0001