Orthogonal nanoarchitectonics of M13 phage for receptor targeted anticancer photodynamic therapy. Issue 3 (18th November 2021)

• Record Type: Journal Article
• Authors: Ulfo, Luca; Cantelli, Andrea; Petrosino, Annapaola; Costantini, Paolo Emidio; Nigro, Michela; Starinieri, Francesco; Turrini, Eleonora; Zadran, Suleman Khan; Zuccheri, Giampaolo; Saporetti, Roberto; Di Giosia, Matteo; Danielli, Alberto; Calvaresi, Matteo
• Is Part Of: Nanoscale. Volume 14:Issue 3(2022)
• Page Start: 632
• Page End: 641
• Publication Date: 2021-11-18
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/d1nr06053h
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : Not all viruses are bad . We developed an orthogonal approach (genetic/chemical) to engineer M13 bacteriophages as targeted vectors for efficient photodynamic killing of cancer cells. Abstract : Photodynamic therapy (PDT) represents a promising therapeutic modality for cancer. Here we used an orthogonal nanoarchitectonics approach (genetic/chemical) to engineer M13 bacteriophages as targeted vectors for efficient photodynamic killing of cancer cells. M13 was genetically refactored to display on the phage tip a peptide (SYPIPDT) able to bind the epidermal growth factor receptor (EGFR). The refactored M13EGFR phages demonstrated EGFR-targeted tropism and were internalized by A431 cancer cells, that overexpress EGFR. Using an orthogonal approach to the genetic display, M13EGFR phages were then chemically modified, conjugating hundreds of Rose Bengal (RB) photosensitizing molecules on the capsid surface, without affecting the selective recognition of the SYPIPDT peptides. Upon internalization, the M13EGFR –RB derivatives generated intracellularly reactive oxygen species, activated by an ultralow intensity white light irradiation. The killing activity of cancer cells is observed at picomolar concentrations of the M13EGFR phage.